A simple, highly sensitive, accurate and selective method for determination of trace amounts of Cu2+ in water samples was reported. In this paper, Isopropyl 2-[(isopropoxy carbothioyl)disulfanyl]ethanethioate (IIDE) in order to prepare an effective sorbent for the preconcentration and determination of Cu. The sorption capacity of Isopropyl 2-[(isopropoxy carbothioyl)disulfanyl]ethanethioate (IIDE)-modified Octadecane-functionalized nano graphene (OD-G) (IIDE MS) was 82.34 mg.g-1 and the optimum pH for the quantitative recovery of Cu was found as 5.3. The optimum flow rate, sorbent amount and sample volume were 8 mL.min-1, 300 mg and 50 mL, respectively. 10 mL of 0.1 mol.L-1 HCl was the most suitable eluent. The recommended method is simple and reliable for the determination of Cu without any notable matrix effect and successfully applied to environmental water samples. The limit of detection of the proposed method is 7.5 ng per mL. The method was applied to the extraction and recovery of Cu(II) in different water samples. In the present study, we report the application of preconcentration techniques still continues increasingly for trace metal determinations by flame atomic absorption spectrometry (FAAS) for quantification of Cu in Formalin-fixed paraffin-embedded (FFPE) tissues from Liver loggerhead turtles. This method exhibits the superiority in compared to the other adsorption reagents because of the fact that there is no necessity of any complexing reagent and optimum pH of solution presents in acidic media. In this method is relative standard deviation (R.S.D.) of 2.7%.