In this work, the effect of immobilization of bread yeast (Saccharomyces cerevisiae) by sol-gel technique combined with functionalized nanoporous silica was investigated in different weight ratios of silica containing materials (TMOS: LUS-1). The activities of immobilized yeast in days after immobilization were examined. The results showed immobilization maintain the yeast life for a longer time. The functionalization by C18 functional group improved the environmental conditions for yeast life. These results indicate that the immobilization technique in the gel matrix and porous solid is a good system to develop industrial fermentations.