High Biological performance of Silicon Substituted Nano Hydroxyapatite Synthesized in Simulated Body Fluid at 37°C

Document Type: Research Paper


In this work, we report high biological performance of silicon substituted nano hydroxyapatite (nHA) prepared by immersion of calcium phosphate and sodium silicate as precursors in Simulated Body Fluid (SBF) solution for 24, 36, 48 and 72 hrs at 37°C. Characterization and chemical analysis of the synthesized powders were performed by Fourier transform, infrared spectroscopy (FT-IR), X-ray powder diffraction (XRD) and Dispersive x-ray analysis (EDAX). In vitro biocompatibility test was then carried out by using Bone Marrow Stem Cells (BMSCs) as seeding cells. The MTT assays revealed that, silicon substituted nHA enhance proliferation of the cells and cell cultures showed no negative effect on the cell morphology, viability and proliferation.