Document Type: Research Paper
Biological production of silver nanoparticles, due to its high antimicrobial properties and environmental compatibility, can be used as an alternative to antibiotics. The aim of this study was to optimize the green synthesis of silver nanoparticles using fruit extract of Bunium persicum Boiss and investigate the antimicrobial effect of nanoparticles against Escherichia coli and Staphylococcus aureus. In this study, AgNPs were synthesized by B. persicum fruit extract, as a green reducing agent. The color change of the solution is the first indication of the formation of silver nanoparticles. The quality of nanoparticles produced by SEM and UV-Vis spectroscopy was investigated and the average size of nanoparticles was also measured by dynamic light scattering. Antimicrobial effect of synthesized nanoparticles against E. coli and S. aureus was evaluated by disk diffusion and broth microdilution method. The results of this study showed maximum absorbance at 340 nm. SEM showed that AgNPs are spherical and the mean average size of them which is indicated with DLS was 21.3 nm. Minimum inhibitory concentration (MIC) of AgNPs was determined for E. coli 0.78 mg/ml and for S. aureus 1.56 mg/ml. The synthesized silver nanoparticles possess an effective antimicrobial activity against the selected microorganisms.