To study antimicrobial and metal ion potential of Silver nanoparticles synthesized from Zingiber officinale using different solvents by EDS & TEM

Document Type : Research Paper


Nanotechnology is new form of technology which has produced a great development in various
fields. Nanoparticles are of the great scientific interest as they are effectively a bridge between bulk material and atomic & molecular structures. Nanoparticles are the particle that have size 1 to 100 nm and possess due to large surface area to volume ratio & smaller size. Different types of nanomaterial such as Zinc, Copper, Gold, and Silver are available but silver nanoparticles have proven to be most effective. Silver nanoparticles can be synthesized from various conventional methods that are physical, chemical and biological. Physical and
chemical method synthesis is expensive and that have toxic substances. To overcome biological method
provides a feasible alternative. In present study it is reported that a cost effective, simple, environment friendly route of green synthesis of silver nanoparticles using extract of Zingiber officinale prepared from different solvents (double distilled water and 70% ethanol) by hot percolation method. Synthesized Silver nanoparticles were preliminary analysed by using UV-VIS spectrophotometer at 630 nm. Confirmatory analysis
characterization Electron dispersive X-ray spectroscopy (EDS) for presence of true metal ion and Transmission
electron microscopy (TEM) micrographs suggested the size using solvents ddw (double distilled water) 35(±5)
nm and 70% ethanol 50(±5) nm in size. Synthesized Silver nanoparticles were characterized for their
antimicrobial activity against gram positive (Staphylococcus aureus) and gram negative (Escherichia coli,
Pseudomonas aeruginosa) using MIC. Here we purpose the application of synthesized silver nanoparticles from Zingiber officinale for the confirmation of presence of heavy metal ion. Metal nanoparticles have extensively used for presence of metal ion Hg2+ was detected by UV-VIS spectrophotometer at 630 nm.