The immobilization of laccase enzyme from Trametes versicolor on the surface of porous zinc oxide nanoparticles and studying features of the immobilized enzyme

Document Type : Research Paper


The laccase enzyme is the largest group of Oxidoreductase enzymes and is capable of
oxidizing a wide range of organic substrates to water along with molecular oxygen resuscitation. ZnO
nanoparticles are known for their specific properties such as chemical stability, high electrochemical
coupling rates, and wide range of absorption of radiation as multifunctional compounds. In this study,
ZnO porous nanoparticles were synthesized and then the laccase enzyme was stabilized from the source
of vermicellum tramitis by surface absorption method on the surface of synthesized nanoparticles. In
the following, kinetic parameters, temperature stability, reusability and sustainability of the stabilized
enzyme were measured and compared with the free enzyme. Given the results obtained at all three
temperatures (40, 50 and 60 °C), the stabilized enzyme shows more temperature stability than the free
enzyme in desired time range. The kinetic parameters Vm and Km did not significantly change with
respect to the free enzyme. Enzyme activity returned to zero after 10 cycles of use and recycling. While
the free enzyme lost its activity after three weeks of maintenance in laboratory condition, the stabilized
enzyme retained 30% of its initial activity. These results indicate that ZnO porous nanoparticles can be
used as a suitable substrate for the stabilization of laccase enzyme by surface adsorption method, and
improves the stability parameters of the enzyme without affecting the kinetic properties of the enzyme.