Evaluation of antibacterial properties of chitosan nanoparticles against Esherichi coli isolated from diabetic ulcers

Document Type : Research Paper

Abstract

Nanotechnology is the science to create products with enormous potential to treat
diseases. Diabetic ulcers and their infections have affected many people around the world, and many
people die each year from infections caused by microorganisms in these ulcers. The aim of this study
was to investigate the antibacterial properties of chitosan nanoparticles against Escherichia coli isolated
from diabetic wounds. Morphological evaluation and substructure of nanoparticles synthesized by
ion gelling method were performed by XRD and TEM. Investigation of the antibacterial properties of
chitosan nanoparticles on Esherichia coli by qualitative agar well diffusion test and microdilution was
performed to the concentration of 0.064 g/l to 0.256 g/l. The size of chitosan nanoparticles in this study
was 100 nm and spherical shape. The sizes of the inhibition zone were different according to the type
of bacteria and the concentrations of chitosan, the maximum zone diameter 20 mm in concentration
0.256 g.l being observed.

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