Antibacterial activity of Chitosan & ZnO nanoparticles on gram positive and gram-negative bacteria

Document Type : Research Paper


Nanotechnology deals with the manufacture and application of materials with size of up to 100 nm. They are widely used in a number of processes that include material science, agriculture, and food industry, cosmetic, medical, and diagnostic applications. Nanotechnology science can create products with enormous potential to treat all the diseases. Antibacterial substances in nanoparticles are able to fight bacteria and inhibit and slow the growth of bacteria. The aim of this study was to investigate the antibacterial properties of chitosan & ZnO nanoparticles against Escherichia coli & Staphylococcus aureus. Investigation of the antibacterial properties of chitosan& ZnO nanoparticles on Escherichia coli & Staphylococcus aureus by qualitative agar well diffusion test and microdilution was performed to the concentration of 0.032 g/ml to 0.512 g/ml. The size of chitosan & ZnO nanoparticles in this study was 100, 100 nm, and spherical in shape. The sizes of the inhibition zone were different according to the type of bacteria and the concentrations of chitosan& ZnO nanoparticles, the maximum zone diameter 24 and 22 mm in concentration 0.512 g/ml observed.